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RAB3GAP2 Polyclonal antibody

RAB3GAP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24599-1-AP

Synonyms

KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    WB analysis using 24599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24599-1-AP (RAB3GAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human brain using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human brain using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human cerebellum using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using RAB3GAP2 Polyclonal antibody (24599-1-AP)

    IHC staining of human cerebellum using 24599-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24599-1-AP targets RAB3GAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3GAP2 fusion protein Ag20254
Full Name RAB3 GTPase activating protein subunit 2 (non-catalytic)
Calculated Molecular Weight 1393 aa, 156 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC146760
Gene Symbol RAB3GAP2
Gene ID (NCBI) 25782
RRIDAB_2879632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.

Protocols

Product Specific Protocols
WB protocol for RAB3GAP2 antibody 24599-1-APDownload protocol
IHC protocol for RAB3GAP2 antibody 24599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

BING (Verified Customer) (08-04-2021)

A reliable Ab to probe RAB3GAP2 with the right MW of 150 kd.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mouse brain and postmortembrain lysates
RAB3GAP2 Antibody Western Blot validation (1:5000 dilution) in mouse brain and postmortembrain lysates (Cat no:24599-1-AP)