• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of mouse skin tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse skin using 55295-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis using 55295-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis using 55295-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of mouse colon using 55295-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RAB8B Polyclonal antibody (55295-1-AP)

    WB analysis of human brain using 55295-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB8B Polyclonal antibody

RAB8B Polyclonal Antibody for WB, ELISA

Cat No. 55295-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RAB8, Ras related protein Rab 8B, Ras-related protein Rab-8B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inmouse skin tissue, human brain tissue, mouse colon tissue, rat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55295-1-AP targets RAB8B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name RAB8B, member RAS oncogene family
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberNM_016530
Gene Symbol RAB8B
Gene ID (NCBI) 51762
RRIDAB_10896304
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

Protocols

Product Specific Protocols
WB protocol for RAB8B antibody 55295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...