RABEPK Antibody
Rabbit Polyclonal
Catalog number: 10213-2-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 10213-2-AP(RABEPK Antibody) at dilution of 1:800
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | RABEPK fusion protein ag0285 | Full name | Rab9 effector protein with kelch motifs |
|
Calculated molecular weight |
40kd |
Observed molecular
weight |
44kd |
|
GenBank accession number |
BC000503 | Gene ID (NCBI) | 10244 |
| Gene symbol | RABEPK | Synonyms | DKFZp686P1077; RAB9P40; bA65N13.1; p40 |
Background
Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network. |
