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RABGAP1L Polyclonal antibody

RABGAP1L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13894-1-AP

Synonyms

HHL, KIAA0471, RABGAP1L, TBC1D18

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RABGAP1L Polyclonal antibody (13894-1-AP)

    WB analysis of HeLa using 13894-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13894-1-AP (RABGAP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RABGAP1L Polyclonal antibody (13894-1-AP)

    WB analysis of MCF-7 using 13894-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13894-1-AP (RABGAP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RABGAP1L Polyclonal antibody (13894-1-AP)

    IP experiment of HeLa using 13894-1-AP

    IP result of anti-RABGAP1L (IP:13894-1-AP, 4ug; Detection:13894-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using RABGAP1L Polyclonal antibody (13894-1-AP)

    IHC staining of human hepatocirrhosis using 13894-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using RABGAP1L Polyclonal antibody (13894-1-AP)

    IHC staining of human hepatocirrhosis using 13894-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RABGAP1L Polyclonal antibody (13894-1-AP)

    IHC staining of human placenta using 13894-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RABGAP1L Polyclonal antibody (13894-1-AP)

    IHC staining of human placenta using 13894-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13894-1-AP (RABGAP1L antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RABGAP1L Polyclonal antibody (13894-1-AP)

    IF Staining of HeLa using 13894-1-AP

    Immunofluorescent analysis of Hela cells, using RABGAP1L antibody 13894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman hepatocirrhosis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13894-1-AP targets RABGAP1L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABGAP1L fusion protein Ag4856
Full Name RAB GTPase activating protein 1-like
Calculated Molecular Weight 815 aa, 93 kDa
Observed Molecular Weight 93-105 kDa
GenBank Accession NumberBC041888
Gene Symbol RABGAP1L
Gene ID (NCBI) 9910
RRIDAB_10638308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABGAP1L, also known as TBC1D18, is a member of the Tre2/Bub2/Cdc16 (TBC)-domain-containing protein family. Overexpression of RABGAP1L potentiated the antiviral action of IFN against a panel of viruses, including IAV, and its function required a catalytically active TBC domain.

Protocols

Product Specific Protocols
WB protocol for RABGAP1L antibody 13894-1-APDownload protocol
IHC protocol for RABGAP1L antibody 13894-1-APDownload protocol
IF protocol for RABGAP1L antibody 13894-1-APDownload protocol
IP protocol for RABGAP1L antibody 13894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer.

Authors - Emily Golden
View Article
humanWB,IF

Cell Rep

Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.

Authors - Sonja Fernbach
View Article
mouseWB

J Cell Biol

TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1

Authors - Shu Hiragi
  • KD Validated
View Article