Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human endometrial cancer tissue, rat liver tissue, mouse liver tissue, rat testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12735-1-AP targets RabGEF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3442 Product name: Recombinant human RABGEF1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 136-491 aa of BC015330 Sequence: DRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECAQDFYHNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG Predict reactive species |
| Full Name | RAB guanine nucleotide exchange factor (GEF) 1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC015330 |
| Gene Symbol | RABGEF1 |
| Gene ID (NCBI) | 27342 |
| RRID | AB_2175788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJ41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RabGEF1 (Rabex-5) is a guanine nucleotide exchange factor (GEF) for the endocytic pathway regulator Rab5. RabGEF1 contains a Vps9 domain, an A20-like zinc finger (ZnF) domain, and a coiled coil domain (PMID: 17341663). RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It is also implicated in tumorigenesis and in the development of certain human cancers (PMID: 25427001).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| IHC protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| WB protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RabGEF1 Polyclonal antibody (Cat# 12735-1-AP) has 9 citations published in journals including J Clin Invest, ACS Cent Sci, Cell Rep, Front Cell Dev Biol, Sci Rep, FASEB J, Exp Mol Pathol, J Cell Sci, and Orthop Surg. Research fields span lung adenocarcinoma, DNA replication stress, Alzheimer's disease, endosomal trafficking, phagolysosome biology, glioblastoma oncology, endosome formation, and osteoarthritis-related inflammation.
| Species | Application | Title |
|---|---|---|
J Clin Invest Osimertinib activates a TGF-β2-dependent secretory program that drives lung adenocarcinoma progression. | ||
ACS Cent Sci Loss of Diphthamide Increases DNA Replication Stress in Mammalian Cells by Modulating the Translation of RRM1 | ||
Cell Rep Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. | ||
Sci Rep AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway. | ||
FASEB J ATP7A Maintains Bactericidal Function of Neutrophils and Macrophages via Regulating the Formation and Activation of Phagolysosomes |
Reviews
What customers say
One user rated this product 5 stars for Western Blot using hippocampus homogenate at a 1:500 dilution (lot 00003533). They reported it "worked well," indicating reliable performance out of the box. Overall, the limited review base suggests consistent satisfaction among users who have tested it.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kuldeep (Verified Customer) (05-27-2025) | Worked well.
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