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WB analysis using 11588-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11588-1-AP
WB result of RABL2B antibody (11588-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RABL2B transfected HepG2 cells.
WB analysis of human brain using 11588-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11588-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11588-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11588-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of fetal human brain using 11588-1-AP
IP result of anti-RABL2B (IP:11588-1-AP, 4ug; Detection:11588-1-AP 1:300) with fetal human brain tissue lysate 4000 ug.
IHC staining of mouse kidney using 11588-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 11588-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11588-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11588-1-AP (RABL2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11588-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11588-1-AP (RABL2B antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

RABL2B Polyclonal antibody

RABL2B Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11588-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Rab like protein 2B, Rab-like protein 2B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	fetal human brain tissue, HepG2 cells, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, mouse brain tissue
Positive IP detected in	fetal human brain tissue
Positive IHC detected in	mouse kidney tissue, human lung cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

11588-1-AP targets RABL2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2189 Product name: Recombinant human RABL2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC024281 Sequence: MAEDKTKPSELDQGKYDADDNVKIICLGDSAVGKSKLMERFLMDGFQPQQLSTYALTLYKHTATVDGRTILVDFWDTAGQERFQSMHASYYHKAHACIMVFDVQRKVTYRNLSTWYTELREFRPEIPCIVVANKIDADINVTQKSFNFAKKFSLPLYFVSAADGTNVVKLFNDAIRLAVSYKQNSQDFMDEIFQELENFSLEQEEEDVPDQEQSSSIETPSEEAASPHS Predict reactive species
Full Name	RAB, member of RAS oncogene family-like 2B
Calculated Molecular Weight	229 aa, 26 kDa
Observed Molecular Weight	26 kDa
GenBank Accession Number	BC024281
Gene Symbol	RABL2B
Gene ID (NCBI)	11158
RRID	AB_2175954
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UNT1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).

Protocols

Product Specific Protocols
IHC protocol for RABL2B antibody 11588-1-AP	Download protocol
IP protocol for RABL2B antibody 11588-1-AP	Download protocol
WB protocol for RABL2B antibody 11588-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	J Cell Sci RABL2 positively controls localization of GPCRs in mammalian primary cilia. Authors - Izumi Dateyama View Article
human	WB	bioRxiv Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling Authors - Geralle Powell-Rodgers View Article