RAD18 Antibody
Rabbit Polyclonal
Catalog number: 18333-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | IF, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | K-562 cells, A549 cells, HeLa cells, MCF7 cells |
| Recommended dilution | WB: 1:500-1:5000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP(RAD18 Antibody) at dilution of 1:1000
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | RAD18 fusion protein ag12944 | Full name | RAD18 homolog (S. cerevisiae) |
|
Calculated molecular weight |
56kd |
Observed molecular
weight |
75kd,85kd |
|
GenBank accession number |
BC001302 | Gene ID (NCBI) | 56852 |
| Gene symbol | RAD18 | Synonyms | RNF73 |
Background
RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively. Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18. |
