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EPAC1 Polyclonal antibody

EPAC1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, hamster, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12572-1-AP

Synonyms

CAMP GEFI, CGEF1, EPAC, EPAC 1, EPAC1, HSU79275, RAPGEF3, EPAC1

Validation Data Gallery

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  • Western Blot (WB) analysis of CHO cells using EPAC1 Polyclonal antibody (12572-1-AP)

    WB analysis of CHO using 12572-1-AP

    CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EPAC1 Polyclonal antibody (12572-1-AP)

    WB analysis using 12572-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IHC staining of human stomach cancer using 12572-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IHC staining of human stomach cancer using 12572-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IF Staining of mouse testis using 12572-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EPAC1 Polyclonal antibody (12572-1-AP)

    IF Staining of mouse testis using 12572-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCHO cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12572-1-AP targets EPAC1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, hamster, rat samples.

Tested Reactivity human, mouse, hamster, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPAC1 fusion protein Ag3240
Full Name Rap guanine nucleotide exchange factor (GEF) 3
Calculated Molecular Weight 881 aa, 99 kDa
Observed Molecular Weight 99-104 kDa
GenBank Accession NumberBC017728
Gene Symbol RAPGEF3
Gene ID (NCBI) 10411
RRIDAB_11232407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).

Protocols

Product Specific Protocols
WB protocol for EPAC1 antibody 12572-1-APDownload protocol
IHC protocol for EPAC1 antibody 12572-1-APDownload protocol
IF protocol for EPAC1 antibody 12572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tongbin (Verified Customer) (08-25-2020)

This EPAC1 antibody specifically detects EPAC1 in human cell lines.

  • Applications: Western Blot
  • Cell Tissue Type: HEK293T