Filter: AllWBIHCIF/ICC

WB analysis of mouse colon using 19095-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 19095-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 19095-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 19095-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 19095-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 19095-1-AP
Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

RBM19 Polyclonal antibody

Name: RBM19 Polyclonal antibody
Brand: Proteintech
SKU: 19095-1-AP

RBM19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19095-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0682, RBM19, RNA binding motif protein 19

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse colon tissue, COLO 320 cells, mouse small intestine tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19095-1-AP targets RBM19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13545 Product name: Recombinant human RBM19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC006137 Sequence: MSRLIVKNLPNGMKEERFRQLFAAFGTLTDCSLKFTKDGKFRKFGFIGFKSEEEAQKAQKHFNKSFIDTSRITVEFCKSFGDPAKPRAWSKHAQKPSQPKQPPKDSTTPEIKKDEKKKKVAGQLEKLKEDTEFQEFLSVHQRRAQAATWANDGLDAEPSKGKSKPASDYLNFDSDSGQESEEEGAGEDLEEEASLEPKAAVQKELSDMDYLKSKMVKAGSSSSSEEEESEDEAVHCDEGSEAEEEDSSATPVLQERDSRGAGQEQGMPAGKKRPPEARAETEKPANQKEPTTCHTVKLRG Predict reactive species
Full Name	RNA binding motif protein 19
Calculated Molecular Weight	107 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC006137
Gene Symbol	RBM19
Gene ID (NCBI)	9904
RRID	AB_10640906
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4C8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.

Protocols

Product Specific Protocols
IF protocol for RBM19 antibody 19095-1-AP	Download protocol
IHC protocol for RBM19 antibody 19095-1-AP	Download protocol
WB protocol for RBM19 antibody 19095-1-AP	Download protocol