• Featured Product
  • KD/KO Validated

RBM39 Polyclonal antibody

RBM39 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21339-1-AP

Synonyms

CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1



Tested Applications

Positive WB detected inHEK-293T cells, human skeletal muscle tissue, mouse kidney tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21339-1-AP targets RBM39 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM39 fusion protein Ag15584
Full Name RNA binding motif protein 39
Calculated Molecular Weight 530 aa, 59 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC141835
Gene Symbol RBM39
Gene ID (NCBI) 9584
RRIDAB_10733352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

Protocols

Product Specific Protocols
WB protocol for RBM39 antibody 21339-1-APDownload protocol
IHC protocol for RBM39 antibody 21339-1-APDownload protocol
IF protocol for RBM39 antibody 21339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Acta Neuropathol

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Authors - Neumann Manuela M
humanWB

Haematologica

Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.

Authors - Jia Tong
humanWB

J Proteome Res

Proximity Labeling, Quantitative Proteomics, and Biochemical Studies Revealed the Molecular Mechanism for the Inhibitory Effect of Indisulam on the Proliferation of Gastric Cancer Cells.

Authors - Jiaqi Lu
  • KD Validated
humanWB

Cell Rep

Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.

Authors - Tabitha C Ting
humanIHC,RIP

Cell Death Discov

The cancer-testis lncRNA LINC01977 promotes HCC progression by interacting with RBM39 to prevent Notch2 ubiquitination

Authors - Anliang Xia