RBMS2 Monoclonal antibody

RBMS2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67395-1-Ig

Clone No.1E5C5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

1E5C5, RNA-binding motif, single-stranded-interacting protein 2, SCR3, Suppressor of CDC2 with RNA-binding motif 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 67395-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of LNCaP using 67395-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of U2OS using 67395-1-Ig
U2OS cells were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 67395-1-Ig
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse adrenal gland using 67395-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 67395-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	NCI-H1299 cells, U2OS cells, LNCaP cells, A549 cells, HeLa cells
Positive IHC detected in	human placenta tissue, mouse adrenal gland tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67395-1-Ig targets RBMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag27721 Product name: Recombinant human RBMS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-407 aa of BC027863 Sequence: MLLSVTSRPGISTFGYNRNNKKPYVSLAQQMAPPSPSNSTPNSSSGSNGNDQLSKTNLYIRGLQPGTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPSAAQKAVTALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELEGMLKPFGQVISTRILRDTSGTSRGVGFARMESTEKCEAIITHFNGKYIKTPPGVPAPSDPLLCKFADGGPKKRQNQGKFVQNGRAWPRNADMGVMALTYDPTTALQNGFYPAPYNITPNRMLAQSALSPYLSSPVSSYQRVTQTSPLQVPNPSWMHHHSYLMQPSGSVLTPGMDHPISLQPASMMGPLTQQLGHLSLSSTGTYMPTAAAMQGAYISQYTPVPSSSVSVEESSGQQNQVAVDAPSEHGVYSFQFNK Predict reactive species
Full Name	RNA binding motif, single stranded interacting protein 2
Calculated Molecular Weight	407 aa, 44 kDa
Observed Molecular Weight	44 kDa
GenBank Accession Number	BC027863
Gene Symbol	RBMS2
Gene ID (NCBI)	5939
RRID	AB_2882639
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q15434
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBMS2 (RNA-binding motif, single-stranded-interacting protein 2), also known as SCR3 (Suppressor of CDC2 with RNA-binding motif 3), is a member of c-Myc single-strand binding proteins (MSSPs) family and encodes RNA binding protein. RBMS2 plays a critical role in the regulation of transcription and alternative splicing, both key cellular processes related to cell proliferation and programmed cell death or apoptosis (PMID: 40854446). Overexpression of RBMS2 inhibited CRC cell proliferation, migration, and epithelial-mesenchymal transition (EMT) (PMID: 30514345, 38058408).