RBMX2 Polyclonal antibody

RBMX2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17994-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

RNA-binding motif protein, X-linked 2, CGI-79, CGI 79

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis of HepG2 using 17994-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17994-1-AP (RBMX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 17994-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 17994-1-AP (RBMX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HepG2 cells
Positive IF/ICC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

17994-1-AP targets RBMX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12439 Product name: Recombinant human RBMX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC033750 Sequence: MNPLTKVKLINELNEREVQLGVADKVSWHSEYKDSAWIFLGGLPYELTEGDIICVFSQYGEIVNINLVRDKKTGKSKGFCFLCYEDQRSTILAVDNFNGIKIKGRTIRVDHVSNYRAPKDSEEIDDVTRQLQEKGCGARTPSPSLSESSEDEKPTKKHKKDKKEKKKKKKEKEKADREVQAEQPSSSSPRRKTVKEKDDTGPKKHSSKNSERAQKSEPREGQKLPKSRTAYSGGAEDLERELKKEKPKHEHKSSSRREAREEKTRIRDRGRSSDAHSSWYNGRSEGRSYRSRSRSRDKSHRHKRARRSRERESSNPSDRWRH Predict reactive species
Full Name	RNA binding motif protein, X-linked 2
Calculated Molecular Weight	322 aa, 37 kDa
Observed Molecular Weight	30-31 kDa
GenBank Accession Number	BC033750
Gene Symbol	RBMX2
Gene ID (NCBI)	51634
RRID	AB_10641049
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y388
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for RBMX2 antibody 17994-1-AP	Download protocol
WB protocol for RBMX2 antibody 17994-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB,IF	Aging (Albany NY) Aberrant RBMX expression is relevant for cancer prognosis and immunotherapy response Authors - Yilei Sheng View Article
	WB	Nucleic Acids Res A common structural mechanism for RNA recognition by the SF3B complex in mRNA splicing and export Authors - Yuzhu Zhang View Article