Tested Applications
| Positive WB detected in | mouse liver tissue, SGC-7901 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12268-1-AP targets REG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2934 Product name: Recombinant human REG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-158 aa of BC017089 Sequence: VLGDIIMRPSCAPGWFYHKSNCYGYFRKLRNWSDAELECQSYGNGAHLASILSLKEASTIAEYISGYQRSQPIWIGLHDPQKRQQWQWIDGAMYLYRSWSGKSMGGNKHCAEMSSNNNFLTWSSNECNKRQHFLCKYRP Predict reactive species |
| Full Name | regenerating islet-derived family, member 4 |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC017089 |
| Gene Symbol | REG4 |
| Gene ID (NCBI) | 83998 |
| RRID | AB_2178702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYZ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
REG4 (Regenerating islet-derived protein 4) is a secreted C-type lectin that belongs to the regenerating gene (REG) family. Initially identified in a cDNA library from ulcerative colitis tissue, REG4 is predominantly expressed by intestinal enteroendocrine cells and is markedly up-regulated during gut inflammation and in various gastrointestinal cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for REG4 antibody 12268-1-AP | Download protocol |
| WB protocol for REG4 antibody 12268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Rep Med Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers | ||
Cancer Genomics Proteomics Regulation of REG4 Expression and Prediction of 5-Fluorouracil Sensitivity by CDX2 in Ovarian Mucinous Carcinoma. | ||
J Surg Oncol Overexpression of REG4 confers an independent negative prognosticator in rectal cancers receiving concurrent chemoradiotherapy. | ||
Signal Transduct Target Ther Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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