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Tested Applications
| Positive WB detected in | mouse liver tissue, L02 cells |
| Positive IHC detected in | human placenta tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15598-1-AP targets REXO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7976 Product name: Recombinant human REX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-79 aa of BC003502 Sequence: MLGGSLGSRLLRGVGGSHGRFGARGVREGGAAMAAGESMAQRMVWVDLEMTGLDIEKDQIIEMACLITDSDLNILAEGT Predict reactive species |
| Full Name | REX2, RNA exonuclease 2 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC003502 |
| Gene Symbol | REXO2 |
| Gene ID (NCBI) | 25996 |
| RRID | AB_10640529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3B8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for REXO2 antibody 15598-1-AP | Download protocol |
| WB protocol for REXO2 antibody 15598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Vanessa (Verified Customer) (01-11-2021) | Unfortunately, did not obtain a band at the specified size, and could only see a unspecific band see in mitochondrial fractions.
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