RFX3 Polyclonal antibody

RFX3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14784-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Synonyms

Regulatory factor X 3, RFX3, Transcription factor RFX3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIP

WB analysis of HeLa using 14784-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 14784-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 14784-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 14784-1-AP
IP result of anti-RFX3 (IP:14784-1-AP, 4ug; Detection:14784-1-AP 1:500) with HeLa cells lysate 1400ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HeLa cells, K-562 cells
Positive IP detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14784-1-AP targets RFX3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6413 Product name: Recombinant human RFX3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-413 aa of BC067778 Sequence: MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYVEGSDTVYTNGAIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQMGVTGGQLISSSGGTYLIGNSMENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEHKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQQPMQQKQRYKPMQKVDGVADGFTGSGQQTGTSVGQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDIKSLQSLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSTPTDGTTITESRSESTSFPIHFHG Predict reactive species
Full Name	regulatory factor X, 3 (influences HLA class II expression)
Calculated Molecular Weight	84 kDa
Observed Molecular Weight	70-79 kDa
GenBank Accession Number	BC067778
Gene Symbol	RFX3
Gene ID (NCBI)	5991
RRID	AB_2178917
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48380
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IP protocol for RFX3 antibody 14784-1-AP	Download protocol
WB protocol for RFX3 antibody 14784-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
rat	ChIP	Nat Commun Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors. Authors - Karen R Mifsud View Article
mouse	WB	Aging (Albany NY) RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation. Authors - Chan Chen View Article