Tested Applications
| Positive WB detected in | rat lung tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26620-1-AP targets RHOV in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24331 Product name: Recombinant human RHOV protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-236 aa of BC112945 Sequence: SAILSAIEHKARLEKKLNAKGVRTLSRCRWKKFFCFV Predict reactive species |
| Full Name | ras homolog gene family, member V |
| Calculated Molecular Weight | 236 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC112945 |
| Gene Symbol | RHOV |
| Gene ID (NCBI) | 171177 |
| RRID | AB_2880576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96L33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The anti-RHOV antibody (Cat# 26620-1-AP) has 7 total citations published in Cancer Research, International Journal of Biological Sciences, American Journal of Pathology, Frontiers in Oncology, bioRxiv, and Cancer Cell International. Research fields include ovarian cancer peritoneal metastasis, lung adenocarcinoma progression and EGFR-TKI resistance, pancreatic ductal adenocarcinoma, and Rho GTPase signaling pathways. Applications cited are primarily WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Cancer Res RHOV is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal Metastasis.
| ||
Int J Biol Sci RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway. | ||
Am J Pathol Ras Homolog Family Member V Promotes Pancreatic Ductal Adenocarcinoma Progression. | ||
Front Oncol Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma.
| ||
bioRxiv RHOV is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal Metastasis | ||
Cancer Cell Int LLPS-based classification and a novel prognostic signature reveal NRF1 as a therapeutic target in pancreatic cancer. |
