Tested Applications
| Positive WB detected in | BxPC-3 cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11138-1-AP targets RIC8A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1593 Product name: Recombinant human RIC8A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 117-467 aa of BC011821 Sequence: QQLFQELKGVRLLTDTLELTLGVTPEGNPPTLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD Predict reactive species |
| Full Name | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 58-62 kDa |
| GenBank Accession Number | BC011821 |
| Gene Symbol | RIC8A |
| Gene ID (NCBI) | 60626 |
| RRID | AB_2179822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RIC8A antibody 11138-1-AP | Download protocol |
| WB protocol for RIC8A antibody 11138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIC8A Polyclonal antibody (Cat# 11138-1-AP) has a total of 2 citations. These appear in Proceedings of the National Academy of Sciences USA and Frontiers in Cellular Neuroscience. The associated research fields include mitotic spindle orientation and microtubule regulation, as well as G-protein-coupled taste receptor signaling.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation. | ||
Front Cell Neurosci Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling. |
Reviews
What customers say
The single review for 11138-1-AP is from Johnny Zigmond (January 2026), who used it for Western Blot at a 1:2000 dilution on prostate cancer PC3 cell lysate. He rated it 4 out of 5 stars and commented: "Great antibody, minimum non-selective interactions." Overall, the reviewer was satisfied with the antibody's specificity and performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Johnny (Verified Customer) (01-30-2026) | Great antibody, minimum non-selective interactions
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