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RILPL1 Polyclonal antibody

RILPL1 Polyclonal Antibody for WB, ELISA

Cat No. 16732-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWB

WB analysis of HepG2 using 16732-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16732-1-AP (RILPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 16732-1-AP
WB result of RILPL1 antibody (16732-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 16732-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16732-1-AP (RILPL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 16732-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16732-1-AP (RILPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16732-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16732-1-AP (RILPL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16732-1-AP targets RILPL1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10192 Product name: Recombinant human RILPL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC080626 Sequence: MTGMSERERQVMKKLKEVVDKQRDEIRAKDRELGLKNEDVEALQQQQTRLMKINHDLRHRVTVVEAQGKALIEQKVELEADLQTKEQEMGSLRAELGKLRERLQGEHSQNGEEEPETEPVGEESISDAEKVAMDLKDPNRPRFTLQELRDVLHERNELKSKVFLLQEELAYYKSEEMEEENRIPQPPPIAHPRTSPQPESGIKRLFSFFSRDKKRLANTQRNVHIQESFGQWANTHRDDGYTEQGQEALQHL Predict reactive species
Full Name	Rab interacting lysosomal protein-like 1
Calculated Molecular Weight	252 aa, 29 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC080626
Gene Symbol	RILPL1
Gene ID (NCBI)	353116
RRID	AB_2878307
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5EBL4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RILPL1 antibody 16732-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RILPL1 Polyclonal antibody (Cat# 16732-1-AP) has 2 total citations. These appear in the American Journal of Human Genetics and Biochimica et Biophysica Acta General Subjects. The associated research fields include oculopharyngodistal myopathy (a genetic neuromuscular disorder) and colorectal cancer progression via m1A methylation-mediated signaling pathways.

Species	Application	Title
human	IF	Am J Hum Genet The CGG repeat expansion in RILPL1 is associated with oculopharyngodistal myopathy type 4. Authors - Jiaxi Yu View Article
	WB,IHC	Biochim Biophys Acta Gen Subj m1A methylation-mediated upregulation of RILsPL1 promotes colorectal cancer progression via the CaMKII/CREB signaling pathway Authors - Songbo Yang View Article