Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15716-1-AP targets RIMBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8390 Product name: Recombinant human RIMBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC007632 Sequence: MNGLATSLGKGQESAIGGSSAIGEYIRPLPQPGDRPEPLSAKPTFLSRSGSARCRSESDMENERNSNTSKQRYSGKVHLCVARYSYNPFDGPNENPEAELPLTAGKYLYVYGDMDEDGFYEGELLDGQRGLVPSNFVDFVQDNESRLASTLGNEQDQNFINHSGIGLEGEHILDLHSPTHIDAGITDNSAGTLDVNIDDIGEDIVPYPRKITLIKQLAKSVIVGWEPPAVPPGWGTVSSYNVLVDKETRMNLTLGSRTKALIEKLNMAACTYRISVQCVTSRGSSDELQCTLLVGKDVVVAPSHLRVDNITQISAQLSWLPTNSNYSHVIFLNEEEFDIVKAARYKYQFF Predict reactive species |
| Full Name | RIMS binding protein 2 |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 120-150 kDa |
| GenBank Accession Number | BC007632 |
| Gene Symbol | RIMBP2 |
| Gene ID (NCBI) | 23504 |
| RRID | AB_2878173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15034 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The presynaptic active zone mediates synaptic vesicle exocytosis at a synapse. RIM proteins are active zone scaffolding molecules that--among others--mediate vesicle priming and directly or indirectly interact with most other essential presynaptic proteins. RIMBP2 encodes RIMS-binding protein 2 belonging to RIMBP family. RIMBP2 is supposed to be localized at cell membrane especially synaptic plasma membrane. By interacting with RIMS1, RIMS2, CACNA1D and CACNA1B via the SH3 domains, RIMBP2 mediates synaptic transmission as bifunctional linker.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RIMBP2 antibody 15716-1-AP | Download protocol |
| WB protocol for RIMBP2 antibody 15716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIMBP2 Polyclonal antibody (Cat# 15716-1-AP) has 3 total citations. These appear in Cancer Gene Ther and eLife (×2). The cited research spans cancer biology—specifically N6-methyladenosine modification in head and neck squamous cell carcinoma—and cell biology/neuroscience, focusing on Rab27 effector hierarchy in secretory granule exocytosis and Exophilin-8-mediated secretory granule assembly via RIM-BP2 interaction. All citations used the antibody for Western blot (WB) in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Cancer Gene Ther N6-methyladenosine modification of RIMS binding protein 2 promotes head and neck squamous cell carcinoma proliferation and radiotherapy tolerance through endoplasmic reticulum stress | ||
Elife Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.
| ||
Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis |
