Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26111-1-AP targets RIMKLB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23631 Product name: Recombinant human RIMKLB protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-307 aa of BC033793 Sequence: MCSSVAAKLWFLTDRRIREDYPQKEILRALKAKCCEEELDFRAVVMDEVVLTIEQGNLGLRINGELITAYPQVVVVRVPTPWVQSDSDITVLRHLEKMGCRLMNRPQAILNCVNKFWTFQELAGHGVPLPDTFSYGGHENFAKMIDEAEVLEFPMVVKNTRGHRGKAVFLARDKHHLADLSHLIRHEAPYLFQKYVKESHGRDVRVIVVGGRVVGTMLRCSTDGRMQSNCSLGGVGMMCSLSEQGKQLAIQVSNILGMDVCGIDLLMKDDGSFCVCEANANPFQEPKKQIQTNKKIPREKTFLLPPS Predict reactive species |
| Full Name | ribosomal modification protein rimK-like family member B |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42-48 kDa |
| GenBank Accession Number | BC033793 |
| Gene Symbol | RIMKLB |
| Gene ID (NCBI) | 57494 |
| RRID | AB_2880383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULI2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIMKLB antibody 26111-1-AP | Download protocol |
| IHC protocol for RIMKLB antibody 26111-1-AP | Download protocol |
| WB protocol for RIMKLB antibody 26111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RIMKLB polyclonal antibody (Cat# 26111-1-AP) has 3 total citations published in Cell Research, Science Bulletin, and Journal of Inherited Metabolic Disease. The associated research fields include triple-negative breast cancer metabolomics and precision medicine, tumor microenvironment remodeling and breast cancer metastasis, and Canavan disease pathogenesis in mouse models. Applications cited are WB and IHC, with reactivity confirmed in human and mouse.
| Species | Application | Title |
|---|---|---|
Cell Res Comprehensive metabolomics expands precision medicine for triple-negative breast cancer.
| ||
Sci Bull (Beijing) Tumor cell-derived N-acetyl-aspartyl-glutamate reshapes the tumor microenvironment to facilitate breast cancer metastasis
| ||
J Inherit Metab Dis NAAG synthetase deficiency has only low influence on pathogenesis in a Canavan disease mouse model |
Reviews
What customers say
The single review for 26111-1-AP is from a researcher who used it for Western Blot on the Loucy/T-ALL cell line at a 1:1000 dilution. They reported the antibody worked well at the recommended concentration and detected a strong band at approximately 46 kDa. The reviewer gave it a 4 out of 5 rating. Overall, the feedback is positive, confirming reliable performance under standard conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wojciech (Verified Customer) (02-24-2023) | The antibody worked well at the recommended concentration (1:1000). Strong band was detected at ~46 kDa.
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