Tested Applications
| Positive WB detected in | L02 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20093-1-AP targets RIM2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | regulating synaptic membrane exocytosis 2 |
| Calculated Molecular Weight | 160 kDa |
| Observed Molecular Weight | 133 kDa |
| GenBank Accession Number | NM_001100117 |
| Gene Symbol | RIMS2 |
| Gene ID (NCBI) | 9699 |
| RRID | AB_10666429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ26 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIMS2, also named as KIAA0751, RAB3IP3 and RIM2, is a rab effector which involved in exocytosis. RIMS2 may act as scaffold protein. This antibody is specific to RIMS2. It recognizes all the isoforms except isoform7 of RIMS2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RIM2-Specific antibody 20093-1-AP | Download protocol |
| WB protocol for RIM2-Specific antibody 20093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIM2-Specific Polyclonal antibody (Cat# 20093-1-AP) has 3 total citations. These appear in Neuron, Neurobiology of Disease, and bioRxiv. The associated research fields include synaptic transmission and neurotransmitter release machinery (structural integration of presynaptic and postsynaptic components), as well as extracellular vesicle release pathways involving toxic SOD1 oligomers, linking to neurodegeneration studies.
| Species | Application | Title |
|---|---|---|
Neuron Neurexin-1γ drives synaptic transmission through structural integration of neurotransmitter release machinery and postsynaptic receptor nanodomains. | ||
Neurobiol Dis Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers. | ||
bioRxiv Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers |
Reviews
What customers say
The single review for 20093-1-AP is from a researcher who used it for Western Blot on mouse heart tissue (Lot 00012816). They rated it 4 out of 5 stars and reported that the antibody works well for detecting Rims2 in mouse tissue samples. Overall, the feedback is positive, confirming reliable performance in its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-25-2020) | This antibody works well in detecting Rims2 in mouse tissue samples.
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