Tested Applications
| Positive WB detected in | HEK-293T cells, mouse testis tissue, MCF-7 cells, rat testis tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13593-1-AP targets RIOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4497 Product name: Recombinant human RIOK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 215-516 aa of BC039729 Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE Predict reactive species |
| Full Name | RIO kinase 3 (yeast) |
| Calculated Molecular Weight | 516 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC039729 |
| Gene Symbol | RIOK3 |
| Gene ID (NCBI) | 8780 |
| RRID | AB_2178105 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14730 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| IHC protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| IP protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| WB protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RIOK3 antibody (Cat# 13593-1-AP) has 9 total citations across journals including Molecular Cell (3), Cell, Cell Reports, Genes & Development, FEBS Letters, Life Science Alliance, and Biomedicines. Research fields span ribosome biogenesis and quality control, m6A RNA modification, antiviral immune signaling, erythroblast enucleation, glioblastoma cell cycle regulation, ubiquitin chain interactomics, and ratiometric transcriptional activation.
| Species | Application | Title |
|---|---|---|
Mol Cell Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection. | ||
Mol Cell The integrated stress response regulates 18S nonfunctional rRNA decay in mammals | ||
Cell Rep Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.
| ||
Genes Dev miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. | ||
Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. |
Reviews
What customers say
The sole review for this anti-RIOK3 antibody (lot 00079082) is a 5-star rating from a user who tested it by Western Blot at a 1:1000 dilution on HCT116 cells. The reviewer reported good specificity and a clear signal, indicating reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marta (Verified Customer) (07-22-2026) | The anti-RIOK3 antibody worked well for Western blot, showing good specificity and a clear signal.
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