Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, THP-1 cells |
| Positive IP detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15366-1-AP targets RIPK2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0909 Product name: Recombinant human RIPK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-531 aa of BC004553 Sequence: EVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPS Predict reactive species |
| Full Name | receptor-interacting serine-threonine kinase 2 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 50-61 kDa |
| GenBank Accession Number | BC004553 |
| Gene Symbol | RIPK2 |
| Gene ID (NCBI) | 8767 |
| RRID | AB_2253650 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for RIPK2 antibody 15366-1-AP | Download protocol |
| WB protocol for RIPK2 antibody 15366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RIPK2 Antibody (Cat# 15366-1-AP) has 20 citations published across 19 journals, including J Exp Clin Cancer Res, J Neuroinflammation, Cell Death Discov, Adv Healthc Mater, Environ Pollut, Precis Clin Med, Sci Rep, Int Immunopharmacol, and Front Genet. Associated research fields span inflammation, neuroinflammation, pyroptosis/inflammasome activation, NOD/RIPK2/NF-κB signaling, cancer (colorectal, gastric, glioma), gut microbiota dysbiosis, cerebral ischemic injury, cardiac protection, obesity-related insulin resistance, and gouty inflammation.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Unbiased profiling of translational landscape reveals TNFR2 as a translation-dependent vulnerability in colorectal cancer. | ||
J Neuroinflammation H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes | ||
J Neuroinflammation OTUD1 ameliorates cerebral ischemic injury through inhibiting inflammation by disrupting K63-linked deubiquitination of RIP2 | ||
Adv Healthc Mater Transdermal Delivery of Icariin via Microneedle Patches Attenuates Phthalate-Induced Testicular Injury. | ||
Cell Death Discov N4BP3 facilitates NOD2-MAPK/NF-κB pathway in inflammatory bowel disease through mediating K63-linked RIPK2 ubiquitination | ||
Antioxidants (Basel) M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide |
