Filter: AllWBIHCIF-PIF/ICCIF

WB analysis using 15356-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15356-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15356-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 15356-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 15356-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 15356-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15356-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Y79 using 15356-1-AP
Immunofluorescent analysis of (4% PFA) fixed Y79 cells using RLBP1 antibody (15356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of Retinal organoids using 15356-1-AP
Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 15356-1-AP

Publications(19)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (19)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse eye tissue, HepG2 cells, mouse retina tissue, rat eye tissue
Positive IHC detected in	mouse eye tissue, human eye tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse eye tissue, Retinal organoids
Positive IF/ICC detected in	HeLa cells, Y79 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 2 publications below
IF	See 13 publications below

Product Information

15356-1-AP targets RLBP1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7602 Product name: Recombinant human RLBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC004199 Sequence: MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRLQYPELFDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDENILPSDFGGTLPKYDGKAVAEQLFGPQAQAENTAF Predict reactive species
Full Name	retinaldehyde binding protein 1
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC004199
Gene Symbol	RLBP1
Gene ID (NCBI)	6017
RRID	AB_2178530
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12271
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

Protocols

Product Specific Protocols
WB protocol for RLBP1 antibody 15356-1-AP	Download protocol
IHC protocol for RLBP1 antibody 15356-1-AP	Download protocol
IF protocol for RLBP1 antibody 15356-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species Authors - Zhiyang Chen View Article
zebrafish	IF	Aging Cell Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina. Authors - Raquel R Martins View Article
human	WB,IF	Mol Vis Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients Authors - Michael H Farkas View Article
mouse	IF	Diabetes CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Authors - Jose-Andres C Portillo View Article
mouse	WB	FASEB J A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion. Authors - Jose-Andres C Portillo View Article
rat	IF	iScience Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration Authors - Haibin Tian View Article