Tested Applications
| Positive WB detected in | HeLa cells, U2OS cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15606-1-AP targets RNASEH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7990 Product name: Recombinant human RNASEH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC002973 Sequence: MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED Predict reactive species |
| Full Name | ribonuclease H1 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC002973 |
| Gene Symbol | RNASEH1 |
| Gene ID (NCBI) | 246243 |
| RRID | AB_2238624 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60930 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
| IP protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
| WB protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RNASEH1 Polyclonal antibody (Cat# 15606-1-AP) has 63 citations spanning 30 journals, including Nature, Science, Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, and Journal of Clinical Investigation. Associated research fields include R-loop biology, DNA-RNA hybrid processing, genome stability, transcription-replication conflicts, DNA repair, cancer, neurodegeneration, telomere maintenance, and RNA editing.
| Species | Application | Title |
|---|---|---|
Nature EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. | ||
Nature BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
| ||
Science Mechanisms linking cytoplasmic decay of translation-defective mRNA to transcriptional adaptation. | ||
Cell Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy. | ||
Cell Res Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. |
Reviews
What customers say
All four reviewers gave this antibody a perfect 5-star rating. Users report consistent performance across multiple applications including Western blot, immunofluorescence, and immunohistochemistry. Common feedback highlights strong signal and reliable detection. Recommended dilutions range from 1:1000 to 1:3000 for Western blot, 1:250 to 1:200 for IF, and 1:500 for IHC. Tested successfully on HeLa cells and MCF7/mammary gland tissue. Overall, reviewers describe it as an excellent, versatile antibody that works well out of the box.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mulavini (Verified Customer) (09-23-2024) | Works good
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R (Verified Customer) (06-15-2024) | Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells
|
Andrea (Verified Customer) (10-05-2023) | Strong signal, very good detection.
|
Huai-Chin (Verified Customer) (06-08-2019) | Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.
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