Tested Applications
| Positive WB detected in | MCF-7 cells, PC-3 cells, mouse lung tissue, mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17527-1-AP targets RNASEH2B in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11189 Product name: Recombinant human RNASEH2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC036744 Sequence: MAAGVDCGDGVGARQHVFLVSEYLKDASKKMKNGLMFVKLVNPCSGEGAIYLFNMCLQQLFEVKVFKEKHHSWFINQSVQSGGLLHFATPVDPLFLLLHYLIKADKEGKFQPLDQVVVDNVFPNCILLLKLPGLEKLLHHVTEEKGNPEIDNKKYYKYSKEKTLKWLEKKVNQTVAALKTNNVNVSSRVQSTAFFSGDQASTDKEKDYIRYAHGLISDYIPKELSDDLSKYLKLPEPSASLPNPPSKKIKLSDEPVEAKEDYTKFNTKDLKTEKKNSKMTAAQKALAKVDKSGMKSIDTFFGVKKKLERFETLKIKSSKNICFLHVSVCPS Predict reactive species |
| Full Name | ribonuclease H2, subunit B |
| Calculated Molecular Weight | 331 aa, 37 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC036744 |
| Gene Symbol | RNASEH2B |
| Gene ID (NCBI) | 79621 |
| RRID | AB_10597401 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TBB1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASEH2B antibody 17527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNASEH2B Polyclonal antibody (Cat# 17527-1-AP) has 2 citations. They appear in Cell Reports (2024) and Nutrition & Metabolism (2021). The cited research fields include epigenetic regulation of retrotransposition via neddylation inhibition, and metabolic analysis of nonalcoholic fatty liver disease. Both studies used the antibody for Western blot applications, with species tested being human and rat.
| Species | Application | Title |
|---|---|---|
Cell Rep Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition
| ||
Nutr Metab (Lond) Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model. |
