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WB analysis using 16518-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 16518-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16518-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 16518-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 16518-1-AP
IP result of anti-RNASEH2C (IP:16518-1-AP, 3ug; Detection:16518-1-AP 1:500) with HEK-293 cells lysate 3200ug.
IHC staining of human brain using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 16518-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

RNASEH2C Polyclonal antibody

Name: RNASEH2C Polyclonal antibody
Brand: Proteintech
SKU: 16518-1-AP

RNASEH2C Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16518-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, human spleen tissue, human testis tissue, HeLa cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human brain tissue, human lung tissue, human skin tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

16518-1-AP targets RNASEH2C in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9739 Product name: Recombinant human RNASEH2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC023588 Sequence: MESGDEAAIERHRVHLRSATLRDAVPATLHLLPCEVAVDGPAPVGRFFTPAIRQGPEGLEVSFRGRCLRGEEVAVPPGLVGYVMVTEEKKVSMGKPDPLRDSGTDDQEEEPLERDFDRFIGATANFSRFTLWGLETIPGPDAKVRGALTWPSLAAAIHAQVPED Predict reactive species
Full Name	ribonuclease H2, subunit C
Calculated Molecular Weight	164 aa, 18 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC023588
Gene Symbol	RNASEH2C
Gene ID (NCBI)	84153
RRID	AB_2181648
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TDP1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for RNASEH2C antibody 16518-1-AP	Download protocol
IP protocol for RNASEH2C antibody 16518-1-AP	Download protocol
WB protocol for RNASEH2C antibody 16518-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Authors - Michal Zimmermann View Article
human	WB	Hum Mol Genet Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability. Authors - Sara Pizzi View Article
human	WB	J Biol Chem Analysis of ribonucleotide removal from DNA by human nucleotide excision repair. Authors - Laura A Lindsey-Boltz View Article
human	WB	Cell Rep Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition Authors - Yan Li KD Validated View Article
		Cell Rep Guiding ATR and PARP inhibitor combinationswith chemogenomic screens Authors - Michal Zimmermann View Article