Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20219-1-AP targets ROBO1-Specific in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | roundabout, axon guidance receptor, homolog 1 (Drosophila) |
| Calculated Molecular Weight | 181 kDa |
| Observed Molecular Weight | 200-250 kDa |
| GenBank Accession Number | NM_002941 |
| Gene Symbol | ROBO1 |
| Gene ID (NCBI) | 6091 |
| RRID | AB_10694675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6N7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development. The antibody is specific to ROBO1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ROBO1-Specific antibody 20219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ROBO1-Specific Polyclonal antibody (Cat# 20219-1-AP) has 21 citations published across 21 journals, including Nature Cell Biology, Molecular Cell, EMBO Journal, Cell Death & Disease, FASEB Journal, and Development. Research fields span oncology (lung, gastric, breast, glioma, colon, head and neck cancers), neuroscience (axon guidance, neuronal migration), angiogenesis, immunology (psoriasis), developmental biology (placentation, neurodevelopment), fibrosis, diabetic neuropathy, and RNA biology (m6A modification, nonsense-mediated mRNA decay).
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
Mol Cell AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.
| ||
Cell Death Dis Cancer associated fibroblast derived SLIT2 drives gastric cancer cell metastasis by activating NEK9 | ||
Angiogenesis Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment. | ||
Biomater Res Injectable Brain Extracellular Matrix Hydrogels Enhance Neuronal Migration and Functional Recovery After Intracerebral Hemorrhage |
Reviews
What customers say
One customer rated this product 5 stars, using it at a 1:1000 dilution for Western Blot on mouse tissue (lot 00078077). They described it as "best suitable for WB," indicating strong performance and satisfaction with the antibody's specificity and signal quality in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (10-17-2025) | BEST SUITABLE FOR WB
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