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ROGDI Polyclonal antibody

ROGDI Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17047-1-AP

Synonyms

Protein rogdi homolog, ROGDI, rogdi homolog (Drosophila)



Tested Applications

Positive WB detected inhuman brain tissue, human kidney tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17047-1-AP targets ROGDI in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat, Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROGDI fusion protein Ag10688
Full Name rogdi homolog (Drosophila)
Calculated Molecular Weight 287 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC012901
Gene Symbol ROGDI
Gene ID (NCBI) 79641
RRIDAB_2182328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)

Protocols

Product Specific Protocols
WB protocol for ROGDI antibody 17047-1-APDownload protocol
IHC protocol for ROGDI antibody 17047-1-APDownload protocol
IP protocol for ROGDI antibody 17047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Am J Hum Genet

Mutations in ROGDI Cause Kohlschütter-T?nz Syndrome.

Authors - Schossig Anna A
DrosophilaIF

Am J Hum Genet

A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome.

Authors - Mory Adi A
ratWB, IF

Sci Rep

The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein.

Authors - Donatus Riemann
humanWB

Cancer Biol Ther

Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.

Authors - Yi-Fan Chen
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