RPE65 Polyclonal antibody

RPE65 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17939-1-AP

Synonyms

LCA2, mRPE65, rd12, Retinoid isomerohydrolase, Retinol isomerase, RP20, RPE65, sRPE65



Tested Applications

Positive WB detected inY79 cells, HeLa cells, PC-3 cells, mouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17939-1-AP targets RPE65 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPE65 fusion protein Ag12356
Full Name retinal pigment epithelium-specific protein 65kDa
Calculated Molecular Weight 533 aa, 61 kDa
Observed Molecular Weight 65 kDa, 61 kDa
GenBank Accession NumberBC075036
Gene Symbol RPE65
Gene ID (NCBI) 6121
RRIDAB_2285290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

Protocols

Product Specific Protocols
WB protocol for RPE65 antibody 17939-1-APDownload protocol
IHC protocol for RPE65 antibody 17939-1-APDownload protocol
IF protocol for RPE65 antibody 17939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Redox Biol

Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.

Authors - Caiying Liu
WB

J Biol Chem

Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina.

Authors - Zhan Gao
mouseIF

FASEB J

Prph2 disease mutations lead to structural and functional defects in the RPE.

Authors - Lars Tebbe
ratIF

Invest Ophthalmol Vis Sci

Dasatinib Plus Quercetin Alleviates Choroid Neovascularization by Reducing the Cellular Senescence Burden in the RPE-Choroid

Authors - Yinhao Wang
mouseWB

Diabetes Res Clin Pract

Thioredoxin upregulation delays diabetes-induced photoreceptor cell degeneration via AMPK-mediated autophagy and exosome secretion.

Authors - Xiang Ren
ratWB

Am J Pathol

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

Authors - Volha V Malechka

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

  • Applications: Immunofluorescence