Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | A431 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25002-1-AP targets RPL22 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21851 Product name: Recombinant human RPL22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC058887 Sequence: MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED Predict reactive species |
| Full Name | ribosomal protein L22 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15-18 kDa |
| GenBank Accession Number | BC058887 |
| Gene Symbol | RPL22 |
| Gene ID (NCBI) | 6146 |
| RRID | AB_2879841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL22 antibody 25002-1-AP | Download protocol |
| IP protocol for RPL22 antibody 25002-1-AP | Download protocol |
| WB protocol for RPL22 antibody 25002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Cell Death Discov N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness | ||
Carcinogenesis The Molecular Landscape of Synchronous Colorectal Cancer Reveals Genetic Heterogeneity. | ||
Mol Neurobiol Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation | ||
Nat Commun A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yuki (Verified Customer) (01-14-2021) | Cells were grown on sterile coverslips embedded in a 6-well plate. Cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.2% Triton X-100. After blocking with 2% goat serum in PBS, the protein was detected using anti-PRL22 antibodies followed by reaction with Alexa Fluor488- conjugated secondary antibodies.
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