Filter: AllWBIPIHCIF/ICC

WB analysis using 17082-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17082-1-AP (RPL24 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 17082-1-AP
IP result of anti-RPL24 (IP:17082-1-AP, 4ug; Detection:17082-1-AP 1:20000) with HEK-293 cells lysate 920 ug.
IHC staining of human placenta using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17082-1-AP (RPL24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17082-1-AP (RPL24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 17082-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17082-1-AP (RPL24 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 17082-1-AP
Immunofluorescent analysis of Hela cells, using RPL24 antibody 17082-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

RPL24 Polyclonal antibody

Name: RPL24 Polyclonal antibody
Brand: Proteintech
SKU: 17082-1-AP
Rating: 4.00 (1 reviews)

RPL24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17082-1-AP

Publications(21)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

ribosomal protein L24, Large ribosomal subunit protein eL24, 60S ribosomal protein L30, 60S ribosomal protein L24

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (21)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human placenta tissue, human kidney tissue, human liver tissue, human spleen tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 3 publications below
IF	See 5 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

17082-1-AP targets RPL24 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7085 Product name: Recombinant human RPL24 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-157 aa of BC000690 Sequence: MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR Predict reactive species
Full Name	ribosomal protein L24
Calculated Molecular Weight	18 kDa
Observed Molecular Weight	21-23 kDa
GenBank Accession Number	BC000690
Gene Symbol	RPL24
Gene ID (NCBI)	6152
RRID	AB_2181728
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P83731
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]

Protocols

Product Specific Protocols
IF protocol for RPL24 antibody 17082-1-AP	Download protocol
IHC protocol for RPL24 antibody 17082-1-AP	Download protocol
IP protocol for RPL24 antibody 17082-1-AP	Download protocol
WB protocol for RPL24 antibody 17082-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers. Authors - Sridevi Challa KD Validated View Article
human	WB	Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. Authors - Kaosheng Lv View Article
	IF	Neuron Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Authors - Roberta Cagnetta View Article
	WB	Dev Cell Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions Authors - Max Koppers View Article
human	IHC, WB	Proc Natl Acad Sci U S A Local translation in nuclear condensate amyloid bodies. Authors - Phaedra R Theodoridis View Article
	WB,IP	Cell Mol Life Sci Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner Authors - Yonat Tzur View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Erica (Verified Customer) (05-15-2019)

The antibody works very well for WB in both Human HEK and MEF cells. I used 1:2000 dilution in 2%BSA, and it always work well. It doesn't work very well for IP though..