Filter: AllWBIHCIF/ICC

WB analysis of mouse heart using 14826-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse heart using 14826-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse skeletal muscle using 14826-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse skeletal muscle using 14826-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 14826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 14826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
IHC staining of human liver cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14826-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RPL35 Polyclonal antibody

Name: RPL35 Polyclonal antibody
Brand: Proteintech
SKU: 14826-1-AP

RPL35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14826-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ribosomal protein L35, Large ribosomal subunit protein uL29, 60S ribosomal protein L35

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected in	rat pancreas tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

14826-1-AP targets RPL35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6615 Product name: Recombinant human RPL35 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC000348 Sequence: MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVKA Predict reactive species
Full Name	ribosomal protein L35
Calculated Molecular Weight	15 kDa
Observed Molecular Weight	15 kDa
GenBank Accession Number	BC000348
Gene Symbol	RPL35
Gene ID (NCBI)	11224
RRID	AB_2878083
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42766
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for RPL35 antibody 14826-1-AP	Download protocol
IHC protocol for RPL35 antibody 14826-1-AP	Download protocol
WB protocol for RPL35 antibody 14826-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Cell Sci Drp1 regulates transcription of ribosomal protein genes in embryonic hearts. Authors - Qiancong Zhao View Article
monkey	WB	Zool Res Ribosome-associated pathological TDP-43 alters the expression of multiple mRNAs in the monkey brain Authors - Fu-Yu Deng View Article