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Tested Applications
| Positive WB detected in | HepG2 cells, human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
14990-1-AP targets RPL39 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6975 Product name: Recombinant human RPL39 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-51 aa of BC001019 Sequence: MSSHKTFRIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRRHWRRTKLGL Predict reactive species |
| Full Name | ribosomal protein L39 |
| Calculated Molecular Weight | 6 kDa |
| Observed Molecular Weight | 6 kDa |
| GenBank Accession Number | BC001019 |
| Gene Symbol | RPL39 |
| Gene ID (NCBI) | 6170 |
| RRID | AB_2238670 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62891 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL39 antibody 14990-1-AP | Download protocol |
| IHC protocol for RPL39 antibody 14990-1-AP | Download protocol |
| WB protocol for RPL39 antibody 14990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. | ||
Elife In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. | ||
Front Immunol Identification and validation of a 17-gene signature to improve the survival prediction of gliomas
| ||
iScience Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.
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