RPP40 Polyclonal antibody

RPP40 Polyclonal Antibody for WB, ELISA

Cat No. 11736-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RNase P subunit 1, RNaseP protein p40, RNASEP1, RPP40

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of HeLa using 11736-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11736-1-AP (RPP40 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

HeLa cells, COLO 320 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

11736-1-AP targets RPP40 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2341 Product name: Recombinant human RPP40 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC017871 Sequence: MNTGPYYFVKNLPLHELITPEFISTFIKKGKLILSLDKDTYEETGLQGHPSQFSGRKIMKFIVSIDLMELSLNLDSKKYERISWSFKEKKPLKFDFLLAWHKTGSEESTMMSYFSKYQIQEHQPKVALSTLRDLQCPVLQSSELEGTPEVSCRALELFDWLGAVFSNVDLNNEPNNFISTYCCPEPSTVVAKAYLCTITGFILPEKICLLLEHLCHYFDEPKLAPWVTLSVQGFADSPVSWEKK Predict reactive species
Full Name	ribonuclease P/MRP 40kDa subunit
Calculated Molecular Weight	35 kDa, 41 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC017871
Gene Symbol	RPP40
Gene ID (NCBI)	10799
RRID	AB_2182364
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75818
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for RPP40 antibody 11736-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
	WB	Nat Commun Reversible proliferative arrest induced by rapid depletion of RNase MRP Authors - Yuan Liu View Article
		Biochem Biophys Res Commun RPP40, a subunit of Ribonuclease P, facilitates hepatocellular carcinoma proliferation by activating the mTOR/MYC signaling. Authors - Li-na Huang View Article
human	WB	bioRxiv Composition and RNA binding specificity of metazoan RNase MRP Authors - Yuan Liu View Article
		Nucleic Acids Res Composition and RNA binding specificity of metazoan RNase MRP Authors - Yuan Liu View Article