Tested Applications
| Positive WB detected in | HEK-293 cells, mouse testis tissue, PC-12 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11990-1-AP targets RPS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3918 Product name: Recombinant human RPS3 protein Source: e coli.-derived, Unknow Tag: N/A Domain: c16032 of BC034149 Sequence: Predict reactive species |
| Full Name | ribosomal protein S3 |
| Calculated Molecular Weight | 26 aa, 7 kDa |
| Observed Molecular Weight | 26.7 kDa |
| GenBank Accession Number | BC034149 |
| Gene Symbol | RPS3 |
| Gene ID (NCBI) | 6188 |
| RRID | AB_2180758 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS3 antibody 11990-1-AP | Download protocol |
| IHC protocol for RPS3 antibody 11990-1-AP | Download protocol |
| IP protocol for RPS3 antibody 11990-1-AP | Download protocol |
| WB protocol for RPS3 antibody 11990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RPS3 (catalog no. 11990-1-AP) has 33 citations published in journals including Nature Communications, Molecular Cell, EMBO Journal, Cell Reports, Journal of Biological Chemistry, Autophagy, iScience, and Frontiers in Oncology. Research fields span ribosome biology, RNA metabolism, cancer (lung, pancreatic, adenoid cystic carcinoma), DNA repair and genome integrity, viral infections (SARS-CoV-2, KSHV), metabolic disease, reproduction, and inflammation.
| Species | Application | Title |
|---|---|---|
Autophagy Byakangelicin alleviates metabolic dysfunction-associated steatohepatitis by selective inhibition of a non-canonical MTORC1 signaling pathway. | ||
Nat Commun DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death | ||
Nat Commun Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs | ||
Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. | ||
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
Acta Pharm Sin B RBM14 enhances transcriptional activity of p23 regulating CXCL1 expression to induce lung cancer metastasis. |
Reviews
What customers say
Based on 2 reviews, this antibody received an average rating of 4 out of 5. One user (5 stars) reported that it recognized a clean band at 27 kDa in Western Blot. Another user (3 stars) used it at 1:500 dilution on Cl-16 cells for Western Blot but left no specific comments. Overall, the available feedback is limited but positive regarding specificity and band clarity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (12-16-2021) | This antibody recognized a clean band at 27 kd.
|
Huanzhou (Verified Customer) (11-26-2018) |
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