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Tested Applications
| Positive WB detected in | human brain tissue, NIH/3T3 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 6 publications below |
| CoIP | See 1 publications below |
Product Information
17603-1-AP targets RSPH3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11707 Product name: Recombinant human RSPH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-560 aa of BC050604 Sequence: SLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEPGGPGAMTESLEASEFLEQSMSQTRELLLDGGYLQRTTYDRRSSQERKFMEERELLGQDEETAMRKSLGEEELS Predict reactive species |
| Full Name | radial spoke 3 homolog (Chlamydomonas) |
| Calculated Molecular Weight | 560 aa, 64 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC050604 |
| Gene Symbol | RSPH3 |
| Gene ID (NCBI) | 83861 |
| RRID | AB_2181073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RSPH3 antibody 17603-1-AP | Download protocol |
| IHC protocol for RSPH3 antibody 17603-1-AP | Download protocol |
| WB protocol for RSPH3 antibody 17603-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
J Mol Cell Biol Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat. | ||
Andrology Whole-exome sequencing identifies ADGB as a novel causative gene for male infertility in humans: from motility to fertilization | ||
Sci Rep Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice. | ||
J Assist Reprod Genet Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3 mutations have a positive pregnancy outcome following intracytoplasmic sperm injection. | ||
Nat Commun Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia |
