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RSRC1 Polyclonal antibody

RSRC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23826-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Arginine/serine-rich coiled-coil protein 1, BM 011, BM-011, Serine/Arginine-related protein 53, SRrp53

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Freight/Packing: -

Quantity

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Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 23826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23826-1-AP (RSRC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 23826-1-AP
WB result of RSRC1 antibody (23826-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSRC1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 23826-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 23826-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 23826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 23826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 23826-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSRC1 antibody (23826-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human small intestine tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23826-1-AP targets RSRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20604 Product name: Recombinant human RSRC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 172-334 aa of BC006982 Sequence: AGLEHLPPAEQAKARLQLVLEAAAKADEALKAKERNEEEAKRRKEEDQATLVEQVKRVKEIEAIESDSFVQQTFRSSKEVKKSVEPSEVKQATSTSGPASAVADPPSTEKEIDPTSIPTAIKYQDDNSLAHPNLFIEKADAEEKWFKRLIALRQERLMGSPVA Predict reactive species
Full Name	arginine/serine-rich coiled-coil 1
Calculated Molecular Weight	334 aa, 39 kDa
Observed Molecular Weight	38 kDa, 50 kDa
GenBank Accession Number	BC006982
Gene Symbol	RSRC1
Gene ID (NCBI)	51319
RRID	AB_2879329
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q96IZ7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSRC1, also named as SRRP53, is a novel SR-related protein of an apparent molecular mass of 53 kDa (45-53 kDa). It is isolated in a gene trap screen that identifies proteins which localize to the nuclear speckles. RSRC1 plays a rol in pre-mRNA splicing and constitutive.

Protocols

Product Specific Protocols
IF protocol for RSRC1 antibody 23826-1-AP	Download protocol
IHC protocol for RSRC1 antibody 23826-1-AP	Download protocol
WB protocol for RSRC1 antibody 23826-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IHC	Mol Med Rep RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression. Authors - Shijun Yu KD Validated View Article