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WB analysis of HeLa using 17723-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17723-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17723-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17723-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human urothelial carcinoma using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 17723-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17723-1-AP
Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

SAMD4B Polyclonal antibody

SAMD4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17723-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

hSmaug2, Protein Smaug homolog 2, SAM domain-containing protein 4B, Smaug 2, SMAUG2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IHC detected in	human urothelial carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

17723-1-AP targets SAMD4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SAMD4B fusion protein Ag12113 Predict reactive species
Full Name	sterile alpha motif domain containing 4B
Calculated Molecular Weight	694 aa, 75 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC054518
Gene Symbol	SAMD4B
Gene ID (NCBI)	55095
RRID	AB_2183381
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5PRF9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sterile alpha motif domain-containing protein 4B (SAMD4B) is also named as Smaug2. SAMD4B is a mammalian homologue of the Smaug protein of Drosophila melanogaster and can participate in the process of regulating RNA stability as a posttranscriptional inhibitor (PMID: 23592795). SAMD4B is expressed in most human embryos and adult tissue (PMID: 23592795). SAMD4B, especially its expression level, could affect the immune microenvironment (PMID: 38886351).

Protocols

Product Specific Protocols
WB protocol for SAMD4B antibody 17723-1-AP	Download protocol
IHC protocol for SAMD4B antibody 17723-1-AP	Download protocol
IF protocol for SAMD4B antibody 17723-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Mol Immunol SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA. Authors - Yuze Wang KO Validated View Article
human	WB,IF	Mol Med Rep miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B. Authors - Chunrong Wu View Article