Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
22762-1-AP targets SARDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18738 Product name: Recombinant human SARDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 309-430 aa of BC136364 Sequence: NVRDHDASVYLRLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWEVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGGCGQELAHWIIH Predict reactive species |
| Full Name | sarcosine dehydrogenase |
| Calculated Molecular Weight | 918 aa, 101 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC136364 |
| Gene Symbol | SARDH |
| Gene ID (NCBI) | 1757 |
| RRID | AB_2879162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SARDH is a mitochondrial flavoenzyme that oxidizes sarcosine to glycine, feeding one-carbon units into folate metabolism. By tuning methyl-group balance, it modulates epigenetic programs and immune fate. Silencing or mutation of SARDH drives sarcosine accumulation, fueling invasion in prostate, liver, breast and kidney cancers, whereas its high expression predicts favorable prognosis. Thus SARDH acts as a metabolic tumor-suppressor and an emerging therapeutic target. (PMID: 23824605; 40830700; 31545450)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SARDH antibody 22762-1-AP | Download protocol |
| IHC protocol for SARDH antibody 22762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biochem Biophys Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway | ||
Sci Rep Mutation in Smek2 regulating hepatic glucose metabolism causes hypersarcosinemia and hyperhomocysteinemia in rats | ||
Brain Res Brain transcriptomic, metabolic and mitohormesis properties associated with N-propargylglycine treatment: a prevention strategy against neurodegeneration | ||
J Cancer Sarcosine dehydrogenase as an immune infiltration-associated biomarker for the prognosis of hepatocellular carcinoma |
