Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 25729-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25729-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of HeLa using 25729-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
IHC staining of human colon using 25729-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 25729-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 25729-1-AP
Immunofluorescent analysis of HepG2 cells using 25729-1-AP (SERAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SERAC1 Polyclonal antibody

Name: SERAC1 Polyclonal antibody
Brand: Proteintech
SKU: 25729-1-AP

Cat No. 25729-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein SERAC1, SERAC1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	mouse brain tissue, HEK-293 cells, HeLa cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

25729-1-AP targets SERAC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22790 Product name: Recombinant human SERAC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-162 aa of BC001705 Sequence: MSLAAYCVICCRRIGTSTSPPKSGTHWRDIRNIIKFTGSLILGGSLFLTYEVLALKKAVTLDTQVVEREKMKSYIYVHTVSLDKGENHGIAWQARKELHKAVRKVLATSAKILRNPFADPFSTVDIEDHECAVWLLLRKSKSDDKTTRLEAVREMSETHHWH Predict reactive species
Full Name	serine active site containing 1
Calculated Molecular Weight	654 aa, 74 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC001705
Gene Symbol	SERAC1
Gene ID (NCBI)	84947
RRID	AB_2880213
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96JX3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for SERAC1 antibody 25729-1-AP	Download protocol
IHC protocol for SERAC1 antibody 25729-1-AP	Download protocol
WB protocol for SERAC1 antibody 25729-1-AP	Download protocol