Tested Applications
| Positive WB detected in | HeLa cells, human placenta tissue, mouse skeletal muscle tissue, HepG2 cells, MCF-7 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:18000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
14962-1-AP targets SERPINB6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6824 Product name: Recombinant human SERPINB6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC001394 Sequence: MDVLAEANGTFALNLLKTLGKDNSKNVFFSPMSMSCALAMVYMGAKGNTAAQMAQILSFNKSGGGGDIHQGFQSLLTEVNKTGTQYLLRVANRLFGEKSCDFLSSFRDSCQKFYQAEMEELDFISAVEKSRKHINTWVAEKTEGKIAELLSPGSVDPLTRLVLVNAVYFRGNWDEQFDKENTEERLFKVSKNEEKPVQMMFKQSTFKKTYIGEIFTQILVLPYVGKELNMIIMLPDETTDLRTVEKELTYEKFVEWTRLDMMDEEEVEVSLPRFKLEESYDMESVLRNLGMTDAFELGKADFSGMSQTDLSLSKVVHKSFVEVNEEGTEAAAATAAIMMMRCARFVPRFCADHPFLFFIQHSKTNGILFCGRFSSP Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade B (ovalbumin), member 6 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC001394 |
| Gene Symbol | SERPINB6 |
| Gene ID (NCBI) | 5269 |
| RRID | AB_2186456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| IHC protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| IP protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| WB protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Tissue Res Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouse submandibular gland. | ||
Front Neurol Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein. | ||
PLoS One New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. | ||
Cell Mol Gastroenterol Hepatol Single-cell transcriptomics reveals early molecular and immune alterations underlying the serrated neoplasia pathway toward colorectal cancer |
