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Alpha 2-Antiplasmin Polyclonal antibody

Alpha 2-Antiplasmin Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13228-1-AP
Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Alpha-2-antiplasmin, Serpin F2, a2-antiplasmin, A2AP, AAP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of HepG2 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of HepG2 using 13228-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HepG2 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of HepG2 using 13228-1-AP

    WB result of Alpha 2-Antiplasmin antibody (13228-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha 2-Antiplasmin transfected HepG2 cells.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of human liver tissue using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of human liver using 13228-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of human liver tissue using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of human liver using 13228-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of K-562 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of K-562 using 13228-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    IF Staining of HepG2 using 13228-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 2-Antiplasmin antibody (13228-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13228-1-AP targets Alpha 2-Antiplasmin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4095

Product name: Recombinant human SERPINF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 146-491 aa of BC031592

Sequence: HLLSRLCQDLGPGAFRLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDDLANINQWVKEATEGKIQEFLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLTQRDSFHLDEQFTVPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

Predict reactive species
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated Molecular Weight 491 aa, 55 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC031592
Gene Symbol Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2187194
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP08697
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for Alpha 2-Antiplasmin antibody 13228-1-APDownload protocol
WB protocol for Alpha 2-Antiplasmin antibody 13228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

SpeciesApplicationTitle
humanWB

Dis Markers

Identification of differentially expressed serum proteins in infectious purpura fulminans.

Authors - Ting He
View Article
humanWB

Res Pract Thromb Haemost

Citrullination of α2-antiplasmin is unlikely to contribute to enhanced plasmin generation in COVID-19 pathophysiology

Authors - Kadri Kangro
View Article
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