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Alpha 2-Antiplasmin Polyclonal antibody

Alpha 2-Antiplasmin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13228-1-AP

Synonyms

A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, Alpha 2-Antiplasmin, Alpha-2-antiplasmin, API, PLI, Serpin F2, SERPINF2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of HepG2 using 13228-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of human liver using 13228-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of human liver using 13228-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    WB analysis of K-562 using 13228-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 2-Antiplasmin Polyclonal antibody (13228-1-AP)

    IF Staining of HepG2 using 13228-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13228-1-AP targets Alpha 2-Antiplasmin in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha 2-Antiplasmin fusion protein Ag4095
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated Molecular Weight 491 aa, 55 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC031592
Gene Symbol SERPINF2
Gene ID (NCBI) 5345
RRIDAB_2187194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha 2-Antiplasmin antibody 13228-1-APDownload protocol
IF protocol for Alpha 2-Antiplasmin antibody 13228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dis Markers

Identification of differentially expressed serum proteins in infectious purpura fulminans.

Authors - Ting He
View Article
humanWB

Res Pract Thromb Haemost

Citrullination of α2-antiplasmin is unlikely to contribute to enhanced plasmin generation in COVID-19 pathophysiology

Authors - Kadri Kangro
View Article