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SETD6 Polyclonal antibody

SETD6 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 24377-1-AP

Synonyms

SET domain containing 6, SETD6

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using SETD6 Polyclonal antibody (24377-1-AP)

    WB analysis of RAW 264.7 using 24377-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24377-1-AP (SETD6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24377-1-AP is SETD6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETD6 fusion protein Ag19485
Full Name SET domain containing 6
Calculated Molecular Weight 473 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC022451
Gene Symbol SETD6
Gene ID (NCBI) 79918
RRIDAB_2879514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD6 also named as SET domain containing 6 is a 473 amino-acid protein, which localizes in the Nucleus. The molecular weight of SETD6 is 53kDa. SETD6 consists of 1 SET domain and belongs to the class V-like SAM-binding methyltransferase superfamily. SETD6 as a protein-lysine N-methyltransferase that specifically monomethylates at Lysine 310 of the RELA subunit of NF-kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity.

Protocols

Product Specific Protocols
WB protocol for SETD6 antibody 24377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols