SF3B2 Antibody
Rabbit Polyclonal
Catalog number: 10919-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HEK-293 cells, K-562 cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:200-1:1000 IHC: 1:10-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP(SF3B2 Antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human colon cancer using 10919-1-AP(SF3B2 Antibody) at Dilution 1:10 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | SF3B2 fusion protein ag1009 | Full name | splicing factor 3b, subunit 2, 145kDa |
|
Calculated molecular weight |
100kd |
Observed molecular
weight |
130-140kd |
|
GenBank accession number |
BC007610 | Gene ID (NCBI) | 10992 |
| Gene symbol | SF3B2 | Synonyms | SAP145; SF3B145; SF3b1; SF3b150 |
Background
SF3B (splicing factor 3B) is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B complex contains the spliceosome-associated protein (SAP) 49, 130, 145, and 155 has been demonstrated. And this complex is essential for U2 snRNP to anchor to the pre-mRNA. Splicing factor 3B subunit 2 (SF3B2), known as SAP145, forms complex with SAP49 which plays a role in cell cycle progression. SAP145, with predicated MW of 100 kDa, appears with MW of about 145 kDa which may be due to the post-translational modification. |
