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  • KD/KO Validated

ASF/SF2 Polyclonal antibody

ASF/SF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12929-2-AP

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12929-2-AP targets ASF/SF2 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF/SF2 fusion protein Ag3626
Full Name splicing factor, arginine/serine-rich 1
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC010264
Gene Symbol SFRS1
Gene ID (NCBI) 6426
RRIDAB_2187211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

Protocols

Product Specific Protocols
WB protocol for ASF/SF2 antibody 12929-2-APDownload protocol
IHC protocol for ASF/SF2 antibody 12929-2-APDownload protocol
IF protocol for ASF/SF2 antibody 12929-2-APDownload protocol
IP protocol for ASF/SF2 antibody 12929-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Authors - Jing Wen
humanWB

Nat Commun

Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.

Authors - Yuhan Chen
humanWB

Nucleic Acids Res

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Authors - Misbah Khan
mouse,humanIF,WB

Cell Death Differ

The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.

Authors - Jian Sun
humanWB,IF

Angiogenesis

Extracellular matrix stiffness controls VEGF165 secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.

Authors - Min Bao
humanCoIP

EMBO Mol Med

SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity

Authors - Indranil Malik
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Katie (Verified Customer) (03-14-2022)

Works well for western blot - clean blot with one band.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 Western 1:50 IF
  • Cell Tissue Type: EL4 cells / Primary Murine CD4 T cells
FH

Yan (Verified Customer) (04-28-2021)

It worked very well when I performed immunofluorescence. Signal was observed in the nucleus.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Osteosarcoma Epithelial Cells