SFRS2 Polyclonal antibody

SFRS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20371-1-AP

Synonyms

PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2



Tested Applications

Positive WB detected inNIH/3T3 cells, mouse testis tissue, A549 cells, rat brain tissue, HepG2 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20371-1-AP targets SFRS2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name splicing factor, arginine/serine-rich 2
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-35 kDa
GenBank Accession NumberNM_003016
Gene Symbol SFRS2
Gene ID (NCBI) 6427
RRIDAB_2616311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

Protocols

Product Specific Protocols
WB protocol for SFRS2 antibody 20371-1-APDownload protocol
IHC protocol for SFRS2 antibody 20371-1-APDownload protocol
IP protocol for SFRS2 antibody 20371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.

Authors - Yuhan Chen
humanCoIP

EMBO Mol Med

SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity

Authors - Indranil Malik
humanWB

Neuroscience

The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.

Authors - Obayed Raihan
mouseWB

Cell Rep

SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.

Authors - Obayed Raihan
mouseIF

FASEB J

SRSF2 in Sertoli cells is essential for testicular development and spermatogenesis in mice

Authors - Xiaomei Xie
mouseWB

Cell Rep

Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing

Authors - Xuan Gu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xingyuan (Verified Customer) (08-15-2023)

Very nice quality at 1:500 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: hek293t
SFRS2 Antibody Western Blot validation (1:500 dilution) in hek293t (Cat no:20371-1-AP)
FH

Xingyuan (Verified Customer) (07-17-2023)

The quality of SRSF2's antibody wasn't good. I was not able to see bands at around 37kDa (tried 3 times).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
SFRS2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:20371-1-AP)