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WB analysis of HeLa using 19758-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19758-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19758-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19758-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 19758-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 19758-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 19758-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 19758-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 40x lens).

SFT2D3 Polyclonal antibody

Name: SFT2D3 Polyclonal antibody
Brand: Proteintech
SKU: 19758-1-AP

SFT2D3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19758-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

SFT2 domain containing 3, SFT2D3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, human liver tissue
Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19758-1-AP targets SFT2D3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	SFT2 domain containing 3
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	20-22 kDa
GenBank Accession Number	NM_032740
Gene Symbol	SFT2D3
Gene ID (NCBI)	84826
RRID	AB_10640432
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q587I9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFT2D3 belongs to the SFT2 family. SFT2D3 may be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.