Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 15156-1-AP
WB result of SFXN3 antibody (15156-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN3 transfected HeLa cells.
WB analysis using 15156-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15156-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15156-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15156-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 15156-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15156-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15156-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15156-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of BxPC-3 using 15156-1-AP
Immunofluorescent analysis of BxPC-3 cells using 15156-1-AP (SFXN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SFXN3 Polyclonal antibody

Name: SFXN3 Polyclonal antibody
Brand: Proteintech
SKU: 15156-1-AP

SFXN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15156-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Sideroflexin-3, sideroflexin 3, SFX3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	C6 cells, Neuro-2a cells, rat brain tissue, NIH/3T3 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

15156-1-AP targets SFXN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7203 Product name: Recombinant human SFXN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-144 aa of BC000124 Sequence: MESKMGELPLDINIQEPRWDQSTFLGRARHFFTVTDPRNLLLSGAQLEASRNIVQNYRAGVVTPGITEDQLWRAKYVYDSAFHPDTGEKVVLIGRMSAQVPMNMTITGCMLTFYRKTPTVVFWQWVNQSFNAIVNYSNRSGDTP Predict reactive species
Full Name	sideroflexin 3
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC000124
Gene Symbol	SFXN3
Gene ID (NCBI)	81855
RRID	AB_2185825
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWM7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFXN3, a mitochondrial membrane protein, is a member of sideroflexin family. Serum anti-SFXN3 autoantibody was elevated in early stage of the oral squamous cell carcinoma significantly, indicating its clinical value for diagnosis of oral squamous cell carcinoma.

Protocols

Product Specific Protocols
IF protocol for SFXN3 antibody 15156-1-AP	Download protocol
IHC protocol for SFXN3 antibody 15156-1-AP	Download protocol
WB protocol for SFXN3 antibody 15156-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Chem Biol Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers. Authors - Mariluz Soula View Article
human	IHC	PeerJ Identification of iron metabolism-related genes as prognostic indicators for papillary thyroid carcinoma: a retrospective study Authors - Tiefeng Jin View Article
human,mouse	WB	Nucleic Acids Res Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription Authors - Jingke Xie View Article
human	WB	Am J Respir Crit Care Med Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function. Authors - Brian J Sandri View Article