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SGSM1 Polyclonal antibody

SGSM1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21395-1-AP

Synonyms

KIAA1941, RUTBC2, SGSM1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SGSM1 Polyclonal antibody (21395-1-AP)

    WB analysis of mouse testis using 21395-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21395-1-AP (SGSM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SGSM1 Polyclonal antibody (21395-1-AP)

    WB analysis of mouse testis using 21395-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21395-1-AP (SGSM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using SGSM1 Polyclonal antibody (21395-1-AP)

    IHC staining of mouse skin using 21395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21395-1-AP (SGSM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using SGSM1 Polyclonal antibody (21395-1-AP)

    IHC staining of mouse skin using 21395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21395-1-AP (SGSM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21395-1-AP is SGSM1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGSM1 fusion protein Ag16070
Full Name small G protein signaling modulator 1
Calculated Molecular Weight 1148 aa, 130 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC111698
Gene Symbol SGSM1
Gene ID (NCBI) 129049
RRIDAB_10859829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SGSM1 antibody 21395-1-APDownload protocol
IHC protocol for SGSM1 antibody 21395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols